A warm and hearty re-introduction to our newest lab member: Andrew Sue has successfully made the transition from post-bachelor researcher to being our newest Masters Student!
🎉🎉🎉 Congratulations! 🎉🎉🎉
Andrew completed his degree in Biology at SDSU just last year and originally joined us as a lab volunteer. Having had a taste of the phage world, he has decided to pursue a Masters degree with our lab group.
Andrew’s research project will involve the lab’s brand new hypoxic chamber, which capitalizes on some of the research we recently published in Viruses with the Krupovic and Debarbieux groups.
In it we characterized novel phages against C. rodentium, a pathogen for murine intestinal diseases. We also included some preliminary data for a new research direction in the lab—we looked at phage-bacteria interaction (lysis curves) under hypoxic (5% oxygen) conditions to mimic the in vivo environment of the pathogens.
[[Figure 3. CrRp3 or CrRp10 lysis curves of C. rodentium under normoxic and hypoxic conditions. Growth curves of C. rodentium ICC180 infected with CrRp3 (a–e) or CrRp10 (f–j) at MOI ranging between 10–0.001 (solid line) or no phage control (dashed line). Bacteria were growth under either 21% (blue line) or 5% (red line) oxygen and 5% CO2 conditions. N = 6 per condition.]]
Interestingly, under hypoxic conditions we saw that CrRp3 phages at lower MOIs (<0.01) failed to reduce bacterial density below the limit of detection, but that CrRP10 phages were still capable of that reduction.
Look forward to more findings from Andrew and others in the lab that’ll leave you… breathless. 😉